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Coreceptor tropism testing should be performed prior to initiation of therapy with CCR5 inhibitors. HIV‐1 tropism can be assessed with either phenotypic or genotypic methods. The Trofile assay (Monogram Biosciences, South San Francisco, CA) generates pseudovirions from the patient‐derived envelope (env ) …

False positive rates (FPR) were 10% for the proviral DNA sequences obtained in this study or 20% for clonal and clinical data. The Trofile Coreceptor Tropism Assay (Monogram Biosciences, Data on coreceptor tropism in non‐B HIV‐1 subtypes are limited. We studied coreceptor tropism in HIV‐1 circulating in Thailand, where CRF01_AE predominates, using a genotypic assay. Methods. We compiled V3 sequences of HIV‐1 strains circulating in Thailand during 2010–2012. Human immunodeficiency virus (HIV) enters and infects target cells by a process involving interactions between the envelope glycoprotein gp120 and the host cell CD4 receptor, then one or both of two chemokine co-receptors CCR5 and CXCR4.

What is HIV tropism? Tropism refers to the type of cytokine coreceptor used by HIV-1 when infecting the host cell. The viruses in most (>80%) treatment-naïve patients use the CCR5 (R5) coreceptor.1 Conversely, the viruses in up to 50% of treatment-experienced patients use either the CXCR4 (X4) coreceptor or both coreceptors (ie, R5 and X4).2 Viruses that use both coreceptors are Tropism determination: Coreceptor usage was determined using the Geno2pheno [coreceptor] bioinformatics tool (G2P) 10. False positive rates (FPR) were 10% for the proviral DNA sequences obtained in this study or 20% for clonal and clinical data. The Trofile Coreceptor Tropism Assay (Monogram Biosciences, Data on coreceptor tropism in non‐B HIV‐1 subtypes are limited. We studied coreceptor tropism in HIV‐1 circulating in Thailand, where CRF01_AE predominates, using a genotypic assay. Methods.

CD4 cell culture assay for phenotypic recombinant-virus co-receptor tropism. Test Usage Phenotypic determination of the type of co-receptor (CCR5, CXCR4, or both) used by the patient's HIV-1 strain to enter host CD4+ cells (HIV-1 entry co-receptor tropism).

The coreceptor • A co-receptor tropism assay should be performed whenever the use of a CCR5 co-receptor antagonist is being considered (AI). • Co-receptor tropism testing is recommended for patients who exhibit virologic failure on a CCR5 antagonist (BIII). • A phenotypic tropism assay is preferred to determine HIV-1 co-receptor usage (AI).

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Trofile is a highly accurate patient-selection tropism assay that determines which co-receptor an individual's HIV strain uses to enter healthy CD4 cells.

The assay's purpose is to identify the tropism of an individual patient's HIV strain – R5, X4, or a combination of these known as dual/mixed (D/M). The results show whether the patient is infected with virus that enters cells using the R5 co-receptor, the X4 co-receptor, or both (dual/mixed). receptor tropism assays tropism assays Prior art date 2008-03-04 Application number PCT/US2009/001412 Other languages French (fr) Other versions WO2009111042A2 (en Inventor Dwight Dubois Original Assignee Cenetron Diagnostics Priority date (The priority date is an assumption and is not a legal conclusion. Trofile Phenotypic Corecept Tropism Useful For To determine the co-receptor tropism (CCR5, CXCR4, or dual/mixed) of a patient's HIV-1 strain for selection of CCR5 co-receptor antagonist therapy, when a patient's HIV-1 viral load is ≥ 1,000 copies/mL. Trofile HIV co-receptor usage assay. Monogram Trofile has become, largely by default, the de facto standard for HIV tropism assay.
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However, HIV-1 RNA-based tests are not suitable for virologically suppressed patients, therefore the use of proviral DNA tropism testing has been investigated. We describe a novel proviral DNA-based genotypic tropism assay and compare its performance to Trofile Co-Receptor Tropism Assay Useful For Suggests clinical disorders or settings where the test may be helpful. To determine the co-receptor tropism Method Name A short description of the method used to perform the test. CD4 cell culture assay for phenotypic NY State Available Indicates A co-receptor tropism assay should be performed whenever the use of a CCR5 co-receptor antagonist is being considered (AI).

• A genotypic tropism assay should be considered as an alternative … 2015-02-01 Data on coreceptor tropism in non-B HIV-1 subtypes are limited. We studied coreceptor tropism in HIV-1 circulating in Thailand, where CRF01_AE predominates, using a genotypic assay. Methods: We compiled V3 sequences of HIV-1 strains circulating in Thailand during 2010-2012.
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- Tropism testing and treatment history should guide the use of Selzentry With Selzentry's approval, Monogram has launched our latest HIV diagnostic, the Trofile co-receptor tropism assay. Trofile is a highly accurate patient-selection tropism assay that determines which co-receptor an individual's HIV strain uses to enter healthy CD4 cells.

Mayo Test ID; FFTRP: Reporting Name Trofile Phenotypic Corecept Tropism Useful For. To determine the co-receptor tropism (CCR5, CXCR4, or dual/mixed) of a patient's HIV-1 strain for selection of CCR5 co-receptor antagonist therapy, 2013-05-01 View sample report The Trofile Coreceptor Tropism Assay: Is 100% sensitive at detecting 0.3% CXR4-using minor variant.4 Uses the complete gp160 coding region of the HIV-1 envelope protein to ensure that all determinants of tropism are tested. Can successfully amplify and reliably report results with viral loads ≥1,000 copies/mL. genotyping and co-receptor tropism research assay that may in the future help predict the success of antiretroviral agents.

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Co-receptor tropism is defined as an interaction of a virus with a specific co-receptor on the target cell. To gain entry into CD4+ cells, HIV must bind to the cell surface CD4 receptor and to one of two co-receptors, CCR5 or CXCR4.

2015-02-01 · Co-receptor tropism testing is necessary prior to prescribing CCR5 antagonists and plays an important role in the optimal selection of antiretroviral regimens for participants experiencing of virologic failure. A commonly used co-receptor tropism test (Trofile, Monogram Biosciences Inc.) costs $1960 to perform. We studied coreceptor tropism in HIV-1 circulating in Thailand, where CRF01_AE predominates, using a genotypic assay. Methods: We compiled V3 sequences of HIV-1 strains circulating in Thailand during 2010-2012. Coreceptor tropism was predicted based on V3 sequences using geno2pheno version 2.5 (http://coreceptor.bioinf.mpi-inf.mpg.de).